Gel wells were sealed with a small amount of molten agarose and run on the CHEFDr II system (BioRad Laboratories) with the following running parameters: 200 V, 2.
2s initial switching time, 54. 2s final switching time, and 22 h of total running time at 14C. PFGE images were captured, archived, and analyzed using Bionumerics software coupled with the GelDoc 100 System (BioRad). We constructed dendrograms showing percentage of relatedness with use of the Dice coefficient method with 0. 8 optimization and 1. 3 position tolerance. The resulting DNA fragments in plugs were separated by electrophoresis on a 1 agarose (pulsedfield certified agarose, BioRad) gel in 0.
5X TrisborateEDTA (TBE, 45 mM Tris, 4. 5 mM boric acid [pH 8. 3, and 1 mM sodium EDTA) Bio rad pfge manual lawn in a CHEFMapper XA PFGE (BioRad). You have free access to this content Isolation and characterization of bacteriophages specific for Campylobacter jejuni Campylobacter curvus was isolated from blood cultures of a patient with liver abscesses.
Bacterial identification involved Gram staining, biochemical analysis, gasliquid chromatography, and 16S rRNA sequencing. The difficulty in isolation, identification, and growth of the species confirms previous 7Department of Cardiothoracic Surgery, Advocate Christ Medical Center, Oak Lawn, Illinois; 8Department of Thoracic and Cardiovascular Surgery AuroraSt.
Lukes Medical Center, Milwaukee, Wisconsin; and 9 Laboratory of Microbiology, The Rockefeller University, New York, New York, USA Isolation and Characterization of a Generalized Transducing Phage for Pseudomonas aeruginosa Figure Figure1A 1A shows the zones of lysis resulting from DMS3 spotted onto a lawn of (BioRad) in 0.
5 TrisborateEDTA. Electrophoresis was performed in a CHEFDR II apparatus (BioRad) at 14C with a linearly ramped switching time of 1 The strains were grown in TGY and the bacterial pellets incorporated into a final agarose concentration of 1 in PFGE certified agarose (BioRad Laboratories). Plugs were incubated overnight with gentle shaking at 37 C in lysis buffer (0. 5 M EDTA pH8. 0, 2. 5 (vv) of 20 sarkosyl, 0. 25 lysozyme, 0. 2 deoxycholic acid) and subsequently ABSTRACT.
A study of prevalence, diversity, and antimicrobial resistance of Salmonella enterica in surface water in the southeastern United States was conducted. A new scheme was developed for recovery of Salmonella from irrigation pond water and compared with the FDA's Bacteriological Analytical Manual (8th ed.2014) (BAM) Agaroseembedded DNA suitable for separations by field inversion gel electrophoresis (FIGE) or PFGE was prepared in accordance with instructions provided by the manufacturer of the FIGE Mapper electrophoresis system (BioRad Laboratories AG, Glattbrugg, Switzerland) with some modifications.
Subsequently, images were analyzed with the Quantity One software (BioRad Laboratories, Hemel Hempstead, United Kingdom), and the intensity of each band was determined. Genetic typing of parent and derivatives strains by VNTR and PFGE Genome characterization, analysis of virulence and transformation of Microbacterium nematophilum, a coryneform pathogen of the nematode Caenorhabditis elegans Tatiana Akimkina Genetics Unit, Department of Biochemistry, University of Oxford, Oxford, UK