Our school recently purchased a western blot documentation system to start (slowly) moving away from film.
We demo'd the Licor Odyssey, BioRad Chemidoc, and the Syngene Pxi. Simplify your Western blot protocol. Save time and minimize the number of manual steps Licor odyssey western blot manual lawn can introduce variability. NIR fluorescence detection doesnt require chemiluminescent substrates or film.
Imaging Western Blots and Protein Gels on the LICOR Odyssey FC Imager User Guide University of Puget Sound Updated June 2014 by Amy Replogle Table of Contents this is an abbreviated guide.
The actual manual for this imager software package is spread over Refer to V. Adapting Western Blotting Protocols for Odyssey Detection for details. To avoid background speckles on blots, use ultrapure water for buffers LiCor Odyssey Western Blotting. 23, 338 likes 14, 857 talking about this. Be confident in your Western blotting data today and in the future. Partner View and Download LICOR CDiGit user manual online. Blot Scanner. CDiGit Scanner pdf manual download. chemiluminescent detection protocols for Western blotting do not need to be modified for use with the CDiGit Blot Scanner.
Using Blocking Buffer 1. Can I use Odyssey blocking buffer to block my blot? Yes. Use only for the blocking western blot for analysis on licor odyssey scanner.
1) The Licor Odyssey protein marker is optimal as it is visible on channel 700 (2ul is enough for a lane in a 1mm of 10 SDSPolyacrylamide gel). Scientific publishers, funding agencies, and biotechnology and pharmaceutical companies are interested in quantitative Western blots. Journal standards for publishing Western blots have become more rigorous in recent years.
5, 8, 11, 22, 26 Its important to understand the theory and technique of Westerns to get accurate protein Technical Support: or [email protected] com ODYSSEY QuickCard Quantifying Westerns 1. Image Optimization For accurate quantification of a fluorescent western blot, you MUST have background subtraction set.
window within the Odyssey Mar 04, 2014 This video tutorial demonstrates the Image Studio 4. 0 Western Analysis Ribbon, which provides tools to analyze Western blots. These include such functions as Feb 11, 2013 The LICOR CDigit Chemiluminescence Western Blot Scanner from LICOR Biosciences is the latest innovation for developing filmquality images. western blot analysis using the odyssey infrafed imaging system The Odyssey system incorporates fluorescentlylabeled antibodies that can be detected by scanning at the appropriate wavelength.