Lipofectamine 2000 is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats ( e. g. 96well). LIPOFECTAMINE 2000 TRANSFECTION PROTOCOL 10CM PLATES Reagents: 10 FBS in OptiMEM, 1x PBS, OptiMEM, Lipofectamine 2000 Protocol: 1. Thaw FBS. Make sufficient 10 FBS in OptiMEM media (wo antibiotics) sterile filter 2. Aspirate medium off cells 3. Add 23 ml PBS to wash v Experienced Users Procedure Introduction A brief experienced users procedure for transfecting Stealth RNAi or siRNA molecules into mammalian cells using Lipofectamine 2000 Reagent is described below.
Refer to this manual Lipofectamine 2000 is a lipid based transfection reagent for eukaryotic cells. It can be used for transfection of adherent or suspension cells with DNA or RNA (including Stealth RNAi and siRNA) and can be used for either transient Lipofectamine 2000 is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) Use the procedure on page 2 to transfect cells with short interfering RNA (siRNA) or Invitrogen Stealth RNAi.
Use the procedure on page 3 to transfect cells with plasmid DNA. Lipofectamine 2000 is tested for absence of microbial contamination with blood agar plates, Sabaraud dextrose agar plates, and fluid thioglycolate medium, and functionally by transfection of CHOK1 cells with a reporter plasmid.
Lipofectamine 2000 Transfection Reagent tonyzLF2000. pdfThe following citation discusses the effect of variables such as cell density, liposome and DNA concentrations, liposomeDNA complexing time, and media components (serum and antibiotics) on transfection with Lipofectamine Lipofectamine 3000 Reagent Protocol 2000 and Lipofectamine 3000 were used to transfect U2OS and HepG2 cells in a 12well format.
Effi ciency and OFP expression were analyzed 72 hours posttransfection and (A) U2OS and (B) HepG2 Delivery of siRNA for gene knockdown. Lipofectamine Test serumfree media for compatibility with Lipofectamine 2000 Transfection Reagent since some serumfree formulations (e.
g. CD293, SFM II, VPSFM) may inhibit cationic lipidmediated transfection. Lipofectamine 2000 Transfection Reagent is versatile transfection reagent that has been shown to effectively transfect widest variety of adherent and suspension cell lines.
Researchers use Lipofectamine 2000 Reagent for siRNA and shRNAbased gene knockdown experiments, as well as for Lipofectamine 2000 sirna manual expression studies. Lipofectamine 2000 Reagent is a lipidderived reagent designed to deliver exogenic nucleic acids (DNA andor RNA) into eukaryotic cells, either adherent or in suspension. Lipofectamine 2000 Transfection Reagent is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells and provides the following advantages: Highest transfection efficiency in many cell types and formats (